Journal: Biology Open

Article Title: Planar polarization of endogenous ADIP during Xenopus neurulation

doi: 10.1242/bio.062452

Figure Lengend Snippet: Response of endogenous ADIP to wound healing. (A-C) Immunostaining of stage 12 embryos reveals ADIP puncta in ectoderm cells (A). Cell borders are marked by Wtip (A′); merged image (A″) shows partial colocalization of ADIP and Wtip puncta in the apical domain (white arrows). (B) Segmented cell outlines show random orientation of ADIP polarity vectors (arrows). (C) Rose plot confirms random distribution. (D-F) Stage 12 embryos were wounded on the ventral animal side and allowed to heal for 30 min in 0.7× MMR. ADIP becomes polarized in the cells proximal to the wound margin (D). Wtip labels cell borders (D′); merged image (D″). Arrows in D indicate ADIP puncta and polarized Wtip at cell junction facing the wound. Scale bar in D″: 20 μm; the same scale applies to panels A-A′′, B, E, G-G′, H-H′. Cell segmentation (E) and rose plot (F) show ADIP polarity vectors (arrows in E) oriented toward the wound site (0°). Rose plot represents pooled polarity from two embryos. Chi-square test indicates a non-random distribution, P <0.05. (G-I) Anti-Wtip staining of stage 10.5 embryos co-injected with 20 ng of control MO (G-G′) or Wtip MO (H-H′) and 50 pg H2B-GFP RNA as lineage tracer. MO-injected cells are marked by rabbit polyclonal antibody for GFP; merged images in G′,H′. (I) Immunoblotting of endogenous Wtip in stage 13 embryo lysates injected with control MO or Wtip MO. Blots were probed with mouse anti-Wtip (DA2B11) antibody (upper panel) and anti-β-catenin antibody as a loading control (lower panel). Data shown are representative of three independent experiments, each performed on 10-15 embryos.

Article Snippet: Data and resource availability The monoclonal antibody DA2B11 has been deposited in Developmental studies hybridoma bank (DSHB).

Techniques: Immunostaining, Staining, Injection, Control, Western Blot

Journal: Frontiers in Oncology

Article Title: Primary extraovarian adult granulosa cell tumor of the greater omentum: a case report and literature review

doi: 10.3389/fonc.2025.1689815

Figure Lengend Snippet: Pathological characteristics of the extraovarian adult granulosa cell tumor of the greater omentum. (A–D) Photomicrograph of the tumor (H&E stain). (A) The tumor is encapsulated (H&E stain, 40×magnification, Scale bar: 625 μm). (B) Neoplastic cells are arranged in cord-like and ribbon-like patterns (H&E stain, 100×magnification, Scale bar: 200 μm). (C) Call-Exner bodies are visible (H&E stain, 200×magnification, Scale bar: 100 μm). (D) The tumor is composed of round, oval, or polygonal cells with ill-defined cytoplasmic borders imparting a syncytial appearance. The scant cytoplasm ranges from pale to eosinophilic. Nuclei are round, oval, or angular with finely dispersed chromatin and inconspicuous nucleoli. Longitudinal nuclear grooves and coffee-bean shaped nuclei are present(H&E stain, 400×magnification, Scale bar: 50 μm). (E–I) Photomicrograph of immunohistochemical staining. Immunohistochemistry staining is positive for α-inhibin (E) , SF-1 (F) , FOXL2 (G) , WT-1 (H) . (I) Ki-67 Proliferation Index was 20% (IHC stain, 200× magnification, Scale bar: 100 μm).

Article Snippet: WT-1 , Positive , Strong,diffuse nucleus , Supports diagnosis , OTIRIH(Rabbit Monoclonal), Zhongshan Golden Bridge.

Techniques: Staining, Immunohistochemical staining, Immunohistochemistry